Cypress CSC-5300 Uživatelský manuál Strana 1

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Strany 1 - User’s Manual

Nano-Isothermal Titration Calorimeter IIIModel CSC 5300User’s Manual

Strany 2 - CSC 5300 N-ITC III i

4 Chapter 1: Speci cations and Warranty Calorimetry Sciences Corp.CSC 5300 N-ITC III 5 User’s Manualserver) and one person us

Strany 3 - Table of Contents

4 Chapter 1: Speci cations and Warranty Calorimetry Sciences Corp.CSC 5300 N-ITC III 5 User’s Manualor other use expressly pe

Strany 4 - CSC 5300 N-ITC III iii

6 Chapter 1: Speci cations and Warranty Calorimetry Sciences Corp.CSC 5300 N-ITC III 7 User’s ManualThis page intentionally l

Strany 5

6 Chapter 1: Speci cations and Warranty Calorimetry Sciences Corp.CSC 5300 N-ITC III 7 User’s ManualChapter Two: Installation

Strany 6 - CSC 5300 N-ITC III 1

8 Chapter 2: Installation Calorimetry Sciences Corp.CSC 5300 N-ITC III 9 User’s Manualorder to obtain optimum performance from

Strany 7 - Warranty

8 Chapter 2: Installation Calorimetry Sciences Corp.CSC 5300 N-ITC III 9 User’s ManualProduct Items• Model 5300 Nano-Isotherm

Strany 8 - BindWorks Warranty

10 Chapter 2: Installation Calorimetry Sciences Corp.CSC 5300 N-ITC III 11 User’s Manual3. Plug the loose USB cable into the

Strany 9 - CSC 5300 N-ITC III 3

10 Chapter 2: Installation Calorimetry Sciences Corp.CSC 5300 N-ITC III 11 User’s Manual5300 icon located on the desktop or by

Strany 10 - CSC 5300 N-ITC III 5

12 Chapter 2: Installation Calorimetry Sciences Corp.CSC 5300 N-ITC III 13 User’s ManualThis page intentionally left blank.

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12 Chapter 2: Installation Calorimetry Sciences Corp.CSC 5300 N-ITC III 13 User’s ManualChapter Three: Theory of OperationIntr

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CSC 5300 N-ITC III i User’s ManualIsothermal Titration Calorimeter, User’s Manual Revision 40605Copyright ©2005 Calorimetry Sciences

Strany 13 - Chapter Two: Installation

14 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 15 User’s Manualused to determine titrate conce

Strany 14 - N-ITC III Setup

14 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 15 User’s ManualFigure 3-3: Baseline heat outli

Strany 15 - CSC 5300 N-ITC III 9

16 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 17 User’s ManualTitration/Data AnalysisA single

Strany 16

16 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 17 User’s Manualshows that increased overall cu

Strany 17 - CSC 5300 N-ITC III 11

18 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 19 User’s ManualIn order to determine binding c

Strany 18

18 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 19 User’s Manualwhich can then be substituted i

Strany 19 - TED Controlled

20 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 21 User’s Manualsite per protein based on struc

Strany 20 - Applications

20 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 21 User’s ManualFreire, E., Mayorga, O. L. &

Strany 21

22 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 23 User’s ManualThis page intentionally left bl

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22 Chapter 3: Theory of Operation Calorimetry Sciences Corp.CSC 5300 N-ITC III 23 User’s ManualChapter Four: Hardware Descript

Strany 23 - Mathematical Modeling

CSC 5300 N-ITC III i User’s ManualTable of ContentsCHAPTER ONE: SPECIFICATIONS AND WARRANTY. 1Calorimeter Specications ...

Strany 24 - [ ] [ ] [ ] [ ] [ ]( )

24 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 25 User’s ManualFigure 4-1). The power require

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24 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 25 User’s ManualExtreme care should be taken n

Strany 26 - [ ]( ) [ ]( )

26 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 27 User’s Manualrotating shaft of the burette

Strany 27 - Fitting of Calorimetric Data

26 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 27 User’s ManualFigure 4-6: Relative positions

Strany 28

28 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 29 User’s Manualthe calorimeter is the reverse

Strany 29 - CSC 5300 N-ITC III 23

28 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 29 User’s ManualPower SupplyThe calorimeter ha

Strany 30 - CSC 5300 N-ITC III 25

30 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 31 User’s Manualadapter into the sample cell,

Strany 31

30 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 31 User’s Manual5. Apply a vacuum to draw the

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32 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 33 User’s ManualThis page intentionally left b

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32 Chapter 4: Hardware Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 33 User’s ManualChapter Five: Software Descrip

Strany 34

ii Table of Contents Calorimetry Sciences Corp.CSC 5300 N-ITC III iii User’s ManualReinstalling Software...

Strany 35

34 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 35 User’s ManualITCRun will initialize and the

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34 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 35 User’s Manualdata le for review. Also appe

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36 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 37 User’s Manualcommands are described below.E

Strany 38

36 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 37 User’s ManualThe actual injection rate in t

Strany 39 - ITCRun Software Installation

38 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 39 User’s ManualBuret • Move DownThe Buret Mov

Strany 40 - Main Menu

38 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 39 User’s Manualbe used. Click on the stirrer

Strany 41

40 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 41 User’s ManualSetup ButtonUsed to enter the

Strany 42 - Steps =

40 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 41 User’s ManualReset chartThis option complet

Strany 43

42 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 43 User’s Manualon the data tab my be enlarged

Strany 44 - Feature Tabs

42 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 43 User’s Manualslowly to allow air bubbles to

Strany 45 - CSC 5300 N-ITC III 39

ii Table of Contents Calorimetry Sciences Corp.CSC 5300 N-ITC III iii User’s ManualCHAPTER FIVE: SOFTWARE DESCRIPTION ...

Strany 46

44 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 45 User’s Manualthen screw the syringe complet

Strany 47

44 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 45 User’s ManualProgram indicator in ITCRun wi

Strany 48 - CSC 5300 N-ITC III 43

46 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 47 User’s ManualGetting StartedInstalling Bind

Strany 49 - Titrant is Added

46 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 47 User’s Manual2. Select File⇒Open from the

Strany 50

48 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 49 User’s Manual Figure 5-22: Viewing the area

Strany 51 - BindWorks

48 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 49 User’s ManualAs you change the baseline or

Strany 52 - Using BindWorks

50 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 51 User’s ManualThe initial Area display is sh

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50 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 51 User’s ManualModelingModeling OverviewEach

Strany 54

52 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 53 User’s ManualV - is the volume of the cell

Strany 55

52 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 53 User’s ManualRectangular regions represent

Strany 56 - CSC 5300 N-ITC III 51

iv Table of Contents Calorimetry Sciences Corp.CSC 5300 N-ITC III 1 User’s ManualIntegration of the peak ...

Strany 57 - Modeling

54 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 55 User’s ManualCooperative Binding Model This

Strany 58

54 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 55 User’s ManualWhere Xb is the amount of liga

Strany 59 - CSC 5300 N-ITC III 53

56 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 57 User’s ManualIn an experiment designed to m

Strany 60 - Macromolecule

56 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 57 User’s ManualEnter 1 mM for the ligand conc

Strany 61 - Designing an Experiment

58 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 59 User’s ManualThis page intentionally left b

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58 Chapter 5: Software Description Calorimetry Sciences Corp.CSC 5300 N-ITC III 59 User’s ManualChapter Six: Sample Experiment

Strany 63

60 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 61 User’s ManualExperiment Walk-ThroughIntroduct

Strany 64

60 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 61 User’s ManualFigure 6-1: Filling the N-ITC II

Strany 65 - Experiment Overview

62 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 63 User’s Manualand click the burette up icon to

Strany 66 - Chemical Calibration

62 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 63 User’s Manualwhere t is given in °C†.† Christ

Strany 67

iv Table of Contents Calorimetry Sciences Corp.CSC 5300 N-ITC III 1 User’s ManualChapter One: Specications and WarrantyCalori

Strany 68

64 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 65 User’s ManualThe presence of bubbles in the s

Strany 69 - Sample Experiment

64 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 65 User’s ManualBlank TitrationThe heat effect m

Strany 70

66 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 67 User’s Manualprogram main screen (Figure 6-7)

Strany 71

66 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 67 User’s Manualthe tip of the injection needle

Strany 72

68 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 69 User’s ManualThis page intentionally left bla

Strany 73 - CSC 5300 N-ITC III 67

68 Chapter 6: Sample Experiments Calorimetry Sciences Corp.CSC 5300 N-ITC III 69 User’s ManualChapter Seven: TroubleshootingMi

Strany 74

70 Chapter 7: Troubleshooting Calorimetry Sciences Corp.This page intentionally left blank.

Strany 75 - Stirring Speeds

2 Chapter 1: Speci cations and Warranty Calorimetry Sciences Corp.CSC 5300 N-ITC III 3 User’s Manual• Unauthorized modicati

Strany 76

2 Chapter 1: Speci cations and Warranty Calorimetry Sciences Corp.CSC 5300 N-ITC III 3 User’s ManualThis limited warranty giv

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